منابع مشابه
Transformation of yeast by agitation with glass beads.
We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotica...
متن کاملSurface Modification of Glass Beads with an Aminosilane Monolayer
Self-assembled monolayers (SAMs) are monomolecular layers which are spontaneously formed upon immersing a solid substrate into a solution containing functional molecules. The most attractive feature of SAMs is molecular level control over the modification of surfaces, and incorporation of multiple or multilayer molecular components onto the monolayer leads to various functional properties. SAMs...
متن کاملGlass beads load macromolecules into living cells.
We describe and characterize an exceptionally rapid and simple new technique for loading large numbers of cultured cells with large macromolecules. The culture medium of the cell monolayer is replaced by a small volume of the macromolecule to be loaded. Glass beads (75-500 micron diameter) are then sprinkled onto the cells, the cells are washed free of beads and exogenous macromolecules, and 'b...
متن کاملBiologically active catecholamines covalentyly bound to glass beads.
Catecholamines bound covalently to glass beads have been found to have biological activity in several systems. Experimental evidence has been found that immobilized epinephrine and isoproterenol accelerate the heart rate in dogs, chick embryo, and chick heart cells grown in culture, whereas immobilized propranolol results in a decrease in heart rate. Isoproterenol bound to glass beads has also ...
متن کاملA masking analysis of glass pattern perception.
A Glass pattern consists of randomly distributed dot pairs (dipoles) whose orientations are determined by a geometric transform. To understand how an observer perceives the global structure, we investigated how the threshold for detecting a concentric or a radial Glass pattern (target) can be affected by the presence of another Glass pattern (masker). The Glass patterns had either concentric, r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Archaeologia
سال: 1880
ISSN: 0261-3409,2051-3186
DOI: 10.1017/s0261340900007037